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IncuCyte® pHrodo® Red S. aureus Bioparticles® for Phagocytosis

Incucyte® pHrodo® Red S. aureus Bioparticles® for Phagocytosis

One vial of 2 mg sterile lyophilized solid sufficient for 100-200 live cell imaging phagocytosis tests in 96-well format.

(Cat. No. 4619)

Incucyte® pHrodo® Bioparticles® are sterile fluorogenic reagents ideally suited to a simple mix-and-read, real-time live cell quantification of phagocytosis. The unique pHrodo®-based system exploits the acidic environment of the phagosome to quantify phagocytosis. As Incucyte® pHrodo® Bioparticles® residing in the neutral extracellular solution (pH 7.4) are engulfed by phagocytes and enter the acidic phagosome (pH 4.5 – 5.5), a substantial increase in fluorescence is observed. Application of Incucyte® pHrodo® Bioparticles to non-phagocytic cells yields little or no fluorescent signal. With the Incucyte®  integrated analysis software background fluorescence is minimized. These fully sterilized reagents have been validated for use with the Incucyte®  live-cell analysis platform and enable real-time evaluation of phagocytic regulation by pharmacological agents as well as genetic and environmental factors.

Phagocytosis Assay Principle