Incucyte® NucLight Rapid Red Reagent for nuclear labeling
Supplied as a single vial (50 µL), sufficient for 100 to 200 tests in a 96-well plate.
The Incucyte NucLight Rapid Red Reagent for nuclear labeling is a cell permeable DNA stain that specifically stains nuclei in cells and is ideally suited to the mix-and-read, real-time quantification using the Incucyte® Live-Cell Analysis System. Addition of the Incucyte NucLight Rapid Red Reagent to normal healthy cells is non-perturbing to cell growth and morphology and provides homogenous staining of nuclei, enabling the real-time, fluorescent quantification of cell proliferation. When added to tissue culture medium, the inert stain crosses the cell membrane and has excellent specificity for DNA without the need for a wash step. With the Incucyte® integrated analysis software, fluorescent objects can be quantified and background fluorescence minimized. This reagent can be multiplexed with Incucyte® Annexin V, Caspase-3/7 or Cytotox reagents for simultaneous readouts of apoptosis or cytotoxicity alongside cell proliferation.
- Simple to use, mix-and-read reagent
- Rapid, high efficiency cell labeling
- Non-perturbing to cells at optimized dye concentrations
- Ideal for homogenous labeling of multiple cell types
Incucyte NucLight Rapid Red Reagent Quick Guide
NucLight Rapid Red Reagent is non-perturbing and enables high-efficiency labeling of cell nuclei
Optimized dye concentrations do not perturb cell health. NucLight Rapid Red Reagent labeled HT-1080 (adherent; left graph) and Jurkat (non-adherent; right graph) cells show equivalent cell growth profiles to non-labelled cells.
Rapid, high efficiency labelling of adherent and non-adherent cell types. After addition of NucLight Rapid Red Reagent, all cells in the population are labeled immediately and homogenously. Note the homogeneous labeling of cell nuclei and the healthy cell morphology. HT-1080 cells (left image) and Jurkats (right image) show representative nuclear labeling and red fluorescence.
Multiplex with cell health reagents
Analysis of target tumor cell viability over 48 hours multiplexed with an apoptosis readout. HT-1080 cells were labeled with NucLight Rapid Red Reagent and cultured in the presence of Incucyte® Caspase-3/7 Green reagent. Cell death (apoptosis) is monitored in real time by measuring the loss of red object area (left) and increase of green object count (Caspase-3/7 signal) over time.
Visualize changes in the phenotype of labeled immune cells. NucLight Rapid Red labeled human PBMC’s activated with anti-CD3 antibody and IL-2 induces T-cell aggregation or “clustering”. Images shown at are at 0, 60, and 96hrs. User friendly IncuCyte® software enables accurate quantification of cell clustering over time.