IncuCyte® NucLight Green BacMam 3.0 Reagent for nuclear labeling
One vial of BacMam reagent (1 mL; >1 x 108 pfu/mL) suitable for transiently transducing cells with a non-perturbing nuclear restricted green fluorescent label
IncuCyte® NucLight BacMam 3.0 Reagents enable efficient, non-perturbing, nuclear labeling of living mammalian cells. They are compatible with simple mix-and-read protocols, and enable real-time cell counting and the calculation of cell doubling times. IncuCyte® NucLight BacMam 3.0 Reagents enable the rapid expression of a nuclear-restricted GFP (green fluorescent protein) or mKate2 (red fluorescent protein) in your choice of primary, immortalized, dividing or non-dividing mammalian cells without altering cell function and with minimal toxicity. The IncuCyte® NucLight BacMam 3.0 Reagents can be easily used to transiently transduce your choice of cells eliminating the need to create stable cell lines. The NucLight BacMam 3.0 Reagents have been validated for use with the IncuCyte® live-cell analysis system and enable real-time cell counting. Furthermore, the IncuCyte NucLight BacMam 3.0 Reagents can be combined with our range of IncuCyte® Cytotox Reagents, IncuCyte® Annexin V Reagents or the IncuCyte® Caspase-3/7 reagent for multiplexed measurements of cytotoxicity and apoptosis alongside proliferation in a single well.