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Incucyte® NeuroPrime Red Kit

Incucyte® NeuroPrime Red Kit

A complete solution for performing kinetic, live-cell imaging of 96-well co-culture assays utilizing primary rat cortical neurons and astrocytes. Supplied as 1 vial (0.45 mL) Incucyte® NeuroLight Red Lentivirus (synapsin promoter), 1 vial (2 x 106 cells) cryopreserved Incucyte® rCortical Neurons, 1 vial (2 x 106 cells) cryopreserved Incucyte® rAstrocytes.

(Cat. No. 4585)

NeuroPrime Red Kit Contents

The kit is intended for use on Incucyte® Live-Cell Analysis Systems (configured with a green/red optical module), along with the Incucyte® NeuroTrack Analysis Software Module (Cat. No. 9600-0010).

Key Features

  • Cryopreserved vials of cortical neurons and astrocytes isolated from early embryonic stage (E18) Sprague-Dawley rats (1 vial of each, 2 x 106 cells/vial)
  • Incucyte® NeuroLight Red Lentivirus, expressing the red fluorescent protein mKate2 under control of a synapsin promoter (1 vial, 0.45 mL)
  • Minimal expression of fluorescence in non-neuronal cell types
  • The NeuroLight Red Lentivirus is non-perturbing to neuronal biology
  • Validated protocol for optimal neuron labeling and assay performance
  • Ideal for studies of neurotoxicity or general biology of established neural networks

Neuronal Co-Cultures - Fluorescence - Phase, Red and Segmentation