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Incucyte® Neuroactive Orange Kit

Incucyte® Neuroactive Orange Kit

A complete solution for performing kinetic, live-cell imaging of 96-well co-culture assays utilizing primary rat cortical neurons and astrocytes. Supplied as 1 vial (2 mL) Incucyte® Neuroburst Orange Lentivirus Reagent (Synapsin promoter) (Cat. No. 4736) , 1 vial (2 x 106 cells) Incucyte® rCortical Neurons (cryopreserved rat cortical neurons) (Cat. No. 4753), 1 vial (2 x 106 cells) Incucyte® rAstrocytes (cryopreserved rat astrocytes) (Cat. No. 4586)
(Cat. No. 4761)

Neuroactive Orange Kit Contents

The kit is intended for use on the Incucyte® S3 Live-Cell Analysis System for Neuroscience (Cat. No. 4763) and the Incucyte® SX5 Live-Cell Analysis System (Cat. No. 4816) for measurements of neuronal activity and functional connectivity with the Incucyte® Neuronal Activity Analysis Software Module (Cat. No. 9600-0032). This kit is NOT compatible with Incucyte instruments configured with the Green/Red Optical Module, e.g. Cat. No. 4647.

Key Features

  • Cryopreserved vials of cortical neurons and astrocytes isolated from early embryonic stage (E18) Sprague-Dawley rats (1x vial of each, 2 x 106 cells/vial)
  • Incucyte® Neuroburst Orange Lentivirus Reagent, expressing the orange fluorescent protein mRuby under control of a synapsin promoter (1x vial, 2 mL)
  • Minimal expression of fluorescence in non-neuronal cell types
  • The Neuroburst Orange Reagent is non-perturbing to neuronal biology
  • Validated protocol for optimal neuron labeling and assay performance
  • Ideal for studies of neuronal activity established neural networks

Neuroactive Kit Protocol Overview

NeuroBurst Orange Quick Guide