The MMP Orange Reagent Kit assesses changes in MMP, an indicator of mitochondrial health. The cell-permeable MMP Orange Reagent accumulates in active mitochondria in proportion to the MMP generated by the electrochemical gradient across the mitochondrial membrane. A drop in MMP denotes a disruption in mitochondrial function and/or integrity and can also be used as an early indicator of apoptosis. This assay kit utilizes two control compounds: FCCP, an uncoupling agent that transports H+ ions across the mitochondrial membrane and results in depolarization (indicated by a decrease in fluorescence), and Oligomycin A, an inhibitor of ATP synthase which results in hyperpolarization (indicated by an increase in fluorescence).
These reagents have been validated for use with the Incucyte® Live-Cell Analysis System configured with an SX5 Metabolism Optical Module, Green/Orange/NIR or Orange/NIR Optical Module and enables the real-time evaluation of mitochondrial membrane potential. The Incucyte MMP Orange Reagent Kit is intended for use with the Incucyte® Cell-by-Cell Analysis Software Module (Cat. No. 9600-0031).
Determine the state of mitochondria in live cells. Addition of MMP Orange Reagent to HeLa cells enable kinetic monitoring of the mitochondrial membrane potential state (A). Histograms reveal shifts in mean fluorescent intensity for control and drug treated wells at 0 and 40hrs, highlighting the change in mitochondrial polarization. Leverage label-free cell counting with the Cell-by-Cell Analysis Software for real -time data of cell proliferation (C) derived from HD phase/fluorescent images (D).