The IncuCyte® Cell Cycle Lentivirus Reagent is a fluorescent, single cassette indicator expressing both the GFP (green fluorescent protein) and mKate2 (red fluorescent protein) to distinguish between cells in the G1 and S/G2/M cell cycle phase without altering cell function. This reagent is ideal for generating stable cell populations or clones using puromycin selection. The IncuCyte Cell Cycle Lentivirus Reagent has been validated for use with the IncuCyte® Live-cell Analysis System for the real-time, integrated analysis pf spatial and temporal patterns of cell cycle dynamics. Cell-cycle analysis can be multiplexed with label-free cell counts using the IncuCyte® Cell-by-Cell Analysis Software Module for quantification of the total cell population to determine percentage of cells within each cell cycle phase.
Visualizing synchronized division of HeLa cells stably expressing IncuCyte® Cell Cycle Red/Green Lentivirus Reagent. Division of HeLa cells stably expressing IncuCyte® Cell Cycle Red/Green Lentivirus Reagent was arrested by treatment with Thymidine (2.5 mM, 0 - 24h), causing cells to accumulate in G2/M (green). Upon removal of the Thymidine block, cells begin to synchronously divide and progress through the cell cycle (24 – 72h). Data shows the % cells expressing each marker peaks in sequence: non-fluorescent (M – G1 transition), red (G1), red + green (G1 – S transition), green (G2/M). Two full cycles of cell division are observed for this cell type between 24 and 72h.