Incucyte® Cytotox Green Dye for counting dead cells
Supplied as five vials (5 µL per vial, 1 mM in DMSO). Each vial sufficient for 100-200 live cell imaging cytotoxicity tests in 96-well format.
Incucyte® Cytotox Green Dye is a highly sensitive cyanine nucleic acid dye ideally suited to a simple mix-and-read, real-time quantification of cell death. Addition of the Incucyte® Cytotox Green Dye to normal healthy cells is non-perturbing to cell growth or morphology and yields little or no intrinsic fluorescent signal. Once cells become unhealthy plasma membrane integrity diminishes, allowing entry of the Incucyte® Cytotox Green Dye and yielding a 100-1000-fold increase in fluorescence upon binding to deoxyribonucleic acid (DNA). With the Incucyte® integrated analysis software fluorescent objects can be quantified and background fluorescence minimized.
These pre-aliquoted reagents have been validated for use with the Incucyte® Live-Cell Analysis System and enable real-time evaluation of cell membrane integrity and cell death in response to pharmacological agents and/or genetic and environmental factors. Furthermore, the Incucyte® Cytotox Dyes can be combined with the Incucyte® confluence metric, our range of Incucyte® Nuclight nuclear labeling reagents, Incucyte® Annexin V Dyes or Incucyte® Caspase- 3/7 Dyes for multiplexed measurements of cytotoxicity alongside cell proliferation and apoptosis in a single well.