Third generation lentiviral-based vectors are commonly used to transfer genetic information to cells for gene therapy and/or research purposes. The IncuCyte® lentiviral-based reagents have been specially designed to efficiently transduce multiple cell types and provide homogenous expression of fluorescent protein across a population of primary or immortalized, dividing or non-dividing cells with low toxicity. Expression of Green Fluorescent Protein (GFP) is cytoplasmic.
Virus Description: 3rd generation HIV-based, VSV-G pseudotyped lentiviral particles encoding a cytoplasmic restricted Green Fluorescent Protein (GFP). Promoter: EF-1 alpha. Selectable Marker: Puromycin. Spectral Properties: Ex (max): 483 nm; Em (max): 506 nm.