Third generation lentiviral-based vectors are commonly used to transfer genetic information to cells for gene therapy and/or research purposes. The IncuCyte® lentiviral-based reagents have been specially designed to efficiently transduce multiple cell types and provide homogenous expression of fluorescent protein across a population of primary or immortalized, dividing or non-dividing cells with low toxicity. Our extensive validation experiments have shown that expression of either cytoplasmic or nuclear restricted GFP (Green Fluorescent Protein) does not negatively alter functional cell biology (e.g. morphology, proliferation, migration, and differentiation). These reagents can be used either transiently or to generate stable cell populations or clones using puromycin selection. The IncuCyte® lentiviral-based fluorescent protein reagents are particularly suited for use with the IncuCyte® live-cell analysis system.
Virus Description: 3rd generation HIV-based, VSV-G pseudotyped lentiviral particles encoding a cytoplasmic restricted Green Fluorescent Protein (GFP). Promoter: CMV. Selectable Marker: none. Spectral Properties: Ex (max): 483 nm; Em (max): 506 nm.