Working with biomatrices such as Collagen-1 or Matrigel® can be challenging, given their pH- and temperature-dependent viscosity and known batch-to-batch variation. The Incucyte® Cell Invasion Kit enables fine temperature control of biomatrices during assay set up, both for low temperatures (4 - 8 °C) to avoid unwanted gelling, and when steadily and uniformly increasing to 37°C to form the 3D gel.
- 2x CoolBox 96F Systems plus 2 x CoolSink 96F - for close temperature control of biomatrices during preparation in flat-bottom microplates.
- 1 x extra CoolSink 96F - placed at 37°C and ensures even heat transfer to each well of the Incucyte® Imagelock plate during the gelling process.
- CoolBox M30 System with CoolRack – ensures controlled thawing of biomatrix aliquots. Rapid or uneven thawing can impair performance and prevent formation of an optically clear and homogeneous 3D gel.
- Incucyte® Cell Migration & Invasion Assays User Manual - A comprehensive guide to our cell migration/invasion assays, including in depth data analysis and detailed example protocols.