Incucyte® Annexin V Green Reagent for apoptosis
One vial of lyophilized solid sufficient for 100-200 live-cell apoptosis tests in 96-well format.
The Incucyte® Annexin V Reagents are highly-selective phosphatidylserine (PS) cyanine fluorescent dyes. Specially formulated, these reagents are ideally suited for real time measurements of apoptosis in living cultures. They are validated for use with the Incucyte® live-cell analysis system and employ simple mix-and-read protocols. Addition of the Incucyte Annexin V Reagents to normal healthy cells is non-perturbing to cell growth and morphology and yields little or no intrinsic fluorescent signal. Once cells become apoptotic plasma membrane PS asymmetry is lost. PS exposure on the extracellular surface enables binding of the Incucyte Annexin V Reagent resulting in a bright and photostable fluorescent signal. With the Incucyte® integrated analysis software fluorescent apoptotic cells can be quantified and background fluorescence minimized.
Incucyte Annexin V Reagents enable real-time evaluation of PS exposure in response to pharmacological agents and/or genetic and environmental factors. Furthermore, the Incucyte Annexin V Reagents can be combined with the Incucyte® confluence metric, Incucyte® Caspase 3/7 Reagent, Incucyte® NucLight nuclear labeling reagents or Incucyte® Cytotox Reagents for multiplexed measurements of apoptosis, cell proliferation or cytotoxicity in every assay well.