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IncuCyte® A549 NucLight Green Cells

Incucyte® Nuclight Green A549 Cells

One cryopreserved vial of A549 cells expressing Incucyte® Nuclight Green Lentivirus (nuclear-labeled GFP) at a density of 1x106 cells/vial.

(Cat. No. 4492)

Each vial contains a stable population of 1 million A549 cells expressing the Incucyte® Nuclight Green fluorescent protein restricted to the nucleus. Parental A549 cells were purchased from ATCC (Cat. No. CCL-185). A549 cells were transduced with the Incucyte® Nuclight Green Lentivirus (Cat. No. 4475; EF1α, puromycin) at an MOI of 3 (TU/cell) in the presence of 8 µg/ml polybrene following the standard infection protocol. This resulted in ≥70% transduction efficiency. 48 hours post infection, the complete population of cells were grown for 3-5 days in complete growth media containing 1 µg/ml puromycin to select for cells expressing Incucyte® Nuclight Green. Incucyte® Nuclight Green expressing cells are maintained in complete media containing 0.5 µg/ml puromycin. Following selection, a panel of validation assays designed to evaluate the effects of nuclear label expression on functional cell biology was completed. These assays include comparisons of cell morphology, growth/proliferation, and migration between stable populations and the parent populations from which they were derived. In addition, all cells in our Incucyte® Nuclight catalog have been certified mycoplasma free by ATCC.