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IncuCyte® pHrodo® Red Cell Labeling Kit for Phagocytosis

IncuCyte® pHrodo® Red Cell Labeling Kit for Phagocytosis

One vial of lyophilized solid sufficient for labeling 5 x107 target cells of choice - suitable for 100-200 live cell imaging phagocytosis assays in 96-well format.

(Cat. No. 4649)

The IncuCyte® pHrodo® Red Cell Labeling Kit enables the labeling of your choice of target cells with a pH-sensitive fluorophore for use in IncuCyte® phagocytosis assays. Each kit includes:

  • One vial of IncuCyte® pHrodo® Red Cell Labeling dye
  • One vial of DMSO used to solubilize the dye
  • One bottle of wash buffer
  • One bottle of labeling buffer

The unique pHrodo®-based cell labeling system exploits the acidic environment of the phagosome to quantify phagocytosis. As IncuCyte® pHrodo® Red labeled cells residing in the neutral extracellular solution (pH 7.4) are engulfed by phagocytes and enter the acidic phagosome (pH 4.5 – 5.5), a substantial increase in fluorescence is observed. In the absence of phagocytes, the fluorescence intensity of the labeled cells remains low. The IncuCyte® pHrodo® Red Cell Labeling Kit has been validated for use with the IncuCyte® Live-Cell Analysis System and enables real-time evaluation of phagocytic regulation by pharmacological agents as well as genetic and environmental factors.


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