IncuCyte® NucLight Green BacMam 3.0 Reagent for nuclear labeling
One vial of BacMam reagent (1 mL; >1 x 108 pfu/mL) suitable for transiently transducing cells with a non-perturbing nuclear restricted green fluorescent label
IncuCyte® NucLight BacMam Green 3.0 Reagent enables efficient, non-perturbing, nuclear labeling of living mammalian cells. It is compatible with a simple mix-and-read protocol, and enables real-time cell counting and the calculation of cell doubling times. IncuCyte® NucLight BacMam Green 3.0 Reagent enables the rapid expression of a nuclear-restricted GFP (green fluorescent protein) in your choice of primary, immortalized, dividing or non-dividing mammalian cells without altering cell function and with minimal toxicity. The IncuCyte® NucLight BacMam Green 3.0 Reagent can be easily used to transiently transduce your choice of cells eliminating the need to create stable cell lines. The NucLight BacMam 3.0 Reagent has been validated for use with the IncuCyte® live-cell analysis system and enable real-time cell counting. Furthermore, the IncuCyte NucLight BacMam Green 3.0 Reagent can be combined with our range of IncuCyte® Cytotox Reagents, IncuCyte® Annexin V Reagents or the IncuCyte® Caspase-3/7 reagent for multiplexed measurements of cytotoxicity and apoptosis alongside proliferation in a single well.