The IncuCyte® pHrodo® Orange Cell Labeling Kit enables the labeling of your choice of target cells with a pH-sensitive fluorophore for use in IncuCyte® phagocytosis assays. It is intended for use with the IncuCyte® S3 Live-Cell Analysis System for Neuroscience (Cat. No. 4763). The IncuCyte S3 for Neuroscience is configured with an Orange/NIR Optical Module. The pHrodo Orange Cell Labeling Kit is NOT compatible with IncuCyte instruments configured with a Green/Red Optical Module, e.g. Cat. No. 4647.
Each kit includes:
- One vial of IncuCyte® pHrodo® Orange Cell Labeling dye
- One vial of DMSO used to solubilize the dye
- One bottle of wash buffer
- One bottle of labeling buffer
The unique pHrodo®-based cell labeling system exploits the acidic environment of the phagosome to quantify phagocytosis. As IncuCyte® pHrodo® Orange labelled cells residing in the neutral extracellular solution (pH 7.4) are engulfed by phagocytes and enter the acidic phagosome (pH 4.5 – 5.5), a substantial increase in fluorescence is observed. In the absence of phagocytes, the fluorescence intensity of the labeled cells remains low. The IncuCyte pHrodo Orange Cell Labeling Kit has been validated for use with the IncuCyte S3 for Neuroscience and enables real-time evaluation of phagocytic regulation by pharmacological agents as well as genetic and environmental factors.