The Incucyte CytoATP Lentivirus Reagent Kit contains lentiviral based live-cell labeling reagents driven by an EF1-α promotor with puromycin selection, resulting in stable expression of a dual fluorescent (cpmEGFP and mKOk) ATP indicator, either ATP binding (CytoATP Lentivirus) or ATP non-binding control (CytoATP Non-binding Control Lentivirus). CytoATP Lentiviruses are highly efficient, yet non-disruptive, labeling reagents of live cells for the detection and quantification of changes in cytoplasmic ATP in mono- and co-culture cell models.
The CytoATP Lentivirus Reagent Kit has been validated for use with the Incucyte® Live-Cell Analysis System configured with the Metabolism Optical Module (Cat. No. 4820) for measurements of dynamic changes in cytoplasmic ATP. The CytoATP Lentivirus Reagent Kit is not compatible with IncuCyte instruments configured with Green/Orange/NIR or Green/Red Optical Modules. The IncuCyte CytoATP Lentivirus Reagent Kit is intended for use with the Incucyte® ATP Software Module (Cat. No. 9600-0033).