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IncuCyte® NeuroPrime Cell Kit

IncuCyte® NeuroPrime Cell Kit

A complete solution for performing kinetic, live-cell imaging of 96-well co-culture assays utilizing primary rat cortical neurons and astrocytes. Supplied as 1 vial (0.45mL) NeuroLight Red Lentivirus reagent (synapsin promoter), 1 vial (2 x 106 cells) NeuroPrime rCortical Neurons (cryopreserved primary rat forebrain), 1 vial (2 x 106 cells) NeuroPrime rAstrocytes (cryopreserved rat astrocytes)

(Cat. No. 4585)

NeuroPrime Cell Kit Contents

  • 1 vial (0.45mL) NeuroLight Red Lentivirus reagent (synapsin promoter)
  • 1 vial (2 x 106 cells) NeuroPrime rCortical Neurons (cryopreserved primary rat forebrain)
  • 1 vial (2 x 106 cells) NeuroPrime rAstrocytes (cryopreserved rat astrocytes)

Validated protocol for setting up a single 96-well co-culture assay.

The kit is intended for use on the IncuCyte® live-cell analysis system with the IncuCyte® NeuroTrack software module (9600-0010) installed.


Key Features

  • Cryopreserved vials of cortical neurons and astrocytes isolated from early embryonic stage (E18) Sprague-Dawley rats (1x vial of each, 2 x 106 cells/vial)
  • IncuCyte® NeuroLight Red Lentivirus reagent, expressing the red fluorescent protein mKate2 under control of a synapsin promoter (1x vial, 0.45 mL)
  • Minimal expression of fluorescence in non-neuronal cell types
  • NeuroLight Red reagent is non-perturbing to neuronal biology
  • Validated protocol for optimal neuron labeling and assay performance
  • Ideal for studies of neurotoxicity or general biology of established neural networks

Example Data

Neuronal Co-Cultures - Fluorescence - Phase, Red and Segmentation


Assay Performance Data

Lower left: Microplate view of neurite length changes over time for all wells in a 96 well plate. Lower right: Assay stability over multiple days; mean neurite length for one column (n=8) of control wells was measured at 288 hours for 13 plates run over a 5 month period. Solid line plots overall average neurite length and dashed lines plot +20% change from average value.

Microplate view of neurite length changes over time for all wells in a 96 well plate